OMP31 of Brucella melitensis 16M impairs the apoptosis of macrophages triggered by TNF-α
نویسندگان
چکیده
منابع مشابه
Isolation, cloning and expression of the Brucella melitensis Omp31 gene
Brucellosis is a zoonotic disease transmitted to humans either from animals or from their products.Although brucellosis can be found worldwide, the Mediterranean Basin, South and Central America, EasternEurope, Asia, Africa, the Caribbean, and the Middle East have currently been listed as high-risk regions. Thegenus Brucella is classified in at least nine species. Brucella melitensis is the glo...
متن کاملOral vaccination with Brucella melitensis WR201 protects mice against intranasal challenge with virulent Brucella melitensis 16M.
Human brucellosis can be acquired from infected animal tissues by ingestion, inhalation, or contamination of conjunctiva or traumatized skin by infected animal products. In addition, Brucella is recognized as a biowarfare threat agent. Although a vaccine to protect humans from natural or deliberate infection could be useful, vaccines presently used in animals are unsuitable for human use. We te...
متن کاملSurvival of a bacterioferritin deletion mutant of Brucella melitensis 16M in human monocyte-derived macrophages.
A bacterioferritin (BFR) deletion mutant of Brucella melitensis 16M was generated by gene replacement. The deletion was complemented with a broad-host-range vector carrying the wild-type bfr gene, pBBR-bfr. The survival and growth of the mutant, B. melitensis PAD 2-78, were similar to those of its parental strain in human monocyte-derived macrophages (MDM). These results suggest that BFR is not...
متن کاملDifferent Dna Extraction Techniques from Brucella melitensis 16M
Detection of Brucella species using nucleic acid techniques requires efficient unbiased DNA extraction procedures. In this study, we compared six protocols for DNA isolation from pure culture of Brucella melitenses 16M and the efficiency of different extraction processes were evaluated using Polymerase Chain Reaction (PCR).These protocols included boiling, the use of CTAB phenol chloroform extr...
متن کاملisolation, cloning and expression of the brucella melitensis omp31 gene
brucellosis is a zoonotic disease transmitted to humans either from animals or from their products.although brucellosis can be found worldwide, the mediterranean basin, south and central america, easterneurope, asia, africa, the caribbean, and the middle east have currently been listed as high-risk regions. thegenus brucella is classified in at least nine species. brucella melitensis is the glo...
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ژورنال
عنوان ژورنال: Experimental and Therapeutic Medicine
سال: 2016
ISSN: 1792-0981,1792-1015
DOI: 10.3892/etm.2016.3655